Hi Everyone!
It has been an incredible week, even without stepping into the OR once while at Banner. Over the past few days, I spent several hours completing the experimental part of my project—the portion concerning tensile strength, at least. I conducted tests that compared how tensile strength varied with the following features of sutures:
- Size. I used only what was available in the office, so to compare different sized sutures, I used 2-0, 3-0, and 5-0 Monocryl.
- Monofilament vs. Multifilament (braided). To compare these I used 4-0 Ethilon (monofilament) and 4-0 Nurolon (braided), both nylon sutures.
- Barbed vs. Regular. V-loc 180 (absorbable, barbed) and Coated Vicryl (regular, braided)
V-loc 180 Sutures!!!!!!! |
To see a spreadsheet of my results, click here. In the beginning of next week I would also like to run some tests on the tensile strength of Stratafix sutures to compare to the results for the V-loc sutures. They are quite similar barbed sutures but provided by a different company, Ethicon. I will also begin to analyze the results gathered from the tensile strength tests. To do so, I will be finding a 95% confidence interval for each type of suture, and compare the different properties to see if there is a statistically significant difference (s/o to Mr. Peacher and our Stats class!)
Next week, I will be starting a new phase of my project. Using sutured tissue, I will be measuring the burst pressure of the tissue samples once they have been closed with either V-loc, Stratafix, or regular sutures. To summarize, burst pressure describes the maximum amount of pressure an inflated object (a pipe, usually) can withstand before the apparatus begins to leak/break/tear. To conduct this experiment, I will be using an arterial line to measure the pressure. It works, generally, like this:
The A-line will be borrowed from the ICU and the setup will be done by hospital tech, who have generously volunteered their time. Thank you so much! On another note, the tissue I will be using to test burst pressure is going to be pig uterus...Apparently this, along with the pig’s foot, can be obtained at your local asian market. Wish me luck :')
Hope that everyone has a great weekend! As colleges finally begin to release admissions decisions as well (!! D:), I hope that everyone receives good news!!!
Hope that everyone has a great weekend! As colleges finally begin to release admissions decisions as well (!! D:), I hope that everyone receives good news!!!
Best,
Vanessa
What an adventure you are having!! Do hospitals typically bring in a pig's uterus or foot for experimentation?
ReplyDeleteIf a suture bursts while in a patient, is surgery required to go in and collect the pieces? How often does this type of emergency situation present itself and what are the warning signs that patients should look for?
The pig's foot was really just for suturing practice--I was told this would be the best kind of tissue to stitch up. As for pig uterus..I'm not sure! Porcine tissue seems common enough but this will be the first time I have seen pig uterus. Roshan's project last year was done with this tissue but it may not be common to use this for hospital experimentation.
DeleteAs you performed more and more trials, did it become more difficult for you to get accurate results? If so, will you continue trial-testing until the results are comparable?
ReplyDeleteAsian market! :D What a great resource!
Good luck!!
Cool stuff Vanessa, are the experiments exciting? Or do they feel a bit demanding for not a huge reward?
ReplyDeleteNeat spreadsheet, Vanessa! Are you going to make any fancy graphs after you've collected all your data?
ReplyDeleteGreat Article.... Is there any formula through which we can relate these two terms?
ReplyDeletebursting strength tester
What a great article, this information is really helpful for my life and my project
ReplyDeleteThank you a lot!
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